Evaluation of Adhesion, Growth and Differentiation of Human Umbilical Cord Stem Cells to Osteoblast Cells on PLA Polymeric Scaffolds

Tissue engineering is used for regenerating bioartificial tissues using suitable cells and biocompatible scaffold materials. The present study aims to prepare poly (lactic acid) (PLA) nanoparticles as an extracellular matrix use in bone tissue engineering. For this purpose, the stem originating from human umbilical cord were extracted their adhesion, growth, osteoblastic differentiation on PLA studied. First, nano-scaffold was fabricated by solvent casting/salt leaching method, then its properties including formation, surface properties, porosity, pore size, distribution, biocompatibility, biodegradability evaluated. After extracting mesenchymal (MSCs) of embryos, they cultured scaffold, flow cytometry prove stemness. morphological differentiated attached it investigated scanning electron microscopy (SEM), Alizarin red, Von kossa staining. results SEM 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) tests evaluating biocompatibility showed that has cell growth proliferation. result demonstrated more than 90% expression CD105 CD90 markers (MSCs markers) no CD45 (hematopoietic marker) cell’s surface. images red staining, confirmed calcium deposition containing osteoblasts. This MSCs can adhere, proliferate differentiate (osteoblasts) vitro. Thus, additional experiments should be done vivo.